Raw Materials for MDx Real-Time RT-PCR Premixes
1-sec qRT-PCR Premix [Probe] (Ver.2.0)
- Fast (< 45 minutes) or ultrafast (< 25 minutes) for 40 cycles
- Multiplex: Up to 5-plex probes in a reaction
- Ab-based hot start Taq polymerase
- UDG system: Prevention of carryover contamination
Products
| Cat.No. | Product | Feature | Size |
|---|---|---|---|
| BSQR2 | RealHelix™ 1-sec qRT-PCR Premix [Probe] (Ver. 2.0), Bulk | Custom | |
| BSQRU2S | RealHelix™ 1-sec qRT-PCR Premix [Probe] [UDG System] (V2S), Bulk | UDG System | Custom |
This is the bulk version of RealHelix™ 1-sec qRT-PCR Kit [Probe] (Ver. 2.0).
If you would like to purchase in bulk, please contact sale@nanohelix.net.
RealHelix™ 1-sec qRT-PCR Premix [Probe] (Ver. 2.0) is a 2x premix for qRT-PCR assay using fluorescent probe-based detection. This premix effectively delivers reproducible, reliable detection of up to five RNA targets by fast multiplex in a single tube reaction (FAST: 45 min/40 cycles or ULTRAFAST: 25 min/40 cycles). The combination of enzymes (antibody-inhibited hot-start Taq, reverse transcriptase, RNase inhibitor) and NanoHelix’s unique buffers (including dNTPs, Mg2+, and a stabilizing agent) in the ready-to-use premix provides outstanding speed, specificity, and sensitivity of the real-time assay. Bulk version is premix only. ROX reference dye is available separately upon request.
RealHelix™ 1-sec qRT-PCR Premix [Probe] [UDG System] (V2S) is a 2x premix for qRT-PCR assay using fluorescent probe-based detection. This premix effectively delivers reproducible, reliable detection of up to five RNA targets by fast multiplex in a single tube reaction (FAST: 45 min/40 cycles or ULTRAFAST: 25 min/40 cycles). The combination of enzymes (antibody-inhibited hot-start Taq, reverse transcriptase, RNase inhibitor, heat-labile UDG) and NanoHelix’s unique buffers (including dNTP(U)s, Mg2+, and a stabilizing agent) in the ready-to-use premix provides outstanding speed, specificity, and sensitivity of the real-time assay. The applied UDG system prevents the carryover contamination of products from previous reactions.
